Article

The Knock-In Atlas: A web resource for targeted protein trap by CRISPR/Cas9 in human and mouse cell lines

2025-02-22

Abstract excerpt

Various cell engineering techniques have been developed by leveraging the CRISPR-Cas9 technology, but large-scale resources for targeted gene knock-in are still limited. Here we introduce the Knock-in Atlas, a web resource for gene tagging by fluorescent proteins by inserting artificial exons in target gene introns. To produce knock-in cells efficiently and reproducibly, we carefully chose and catalogued guide RNA...

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Literature Corpus work: 1ae9ec06-2e76-5ec9-9a7c-0a08d4ab85c0
DOI: 10.1101/2025.02.19.638564

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