Article

Rapid generation of homozygous fluorescent knock-in human cells using CRISPR/Cas9 genome editing and validation by automated imaging and digital PCR screening

2021-07-01

Abstract excerpt

<h4>ABSTRACT</h4> We have previously described a protocol for genome engineering of mammalian cultured cancer cells with CRISPR/Cas9 to generate homozygous knock-ins of fluorescent tags into endogenous genes 1 . Here, we are updating this protocol to reflect major improvements in the workflow regarding efficiency and throughput. In brief, we have improved our method by combining high efficiency electroporation o...

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Literature Corpus work: 023d7c87-4747-5f39-9bd4-231db8eab1b2
DOI: 10.1101/2021.06.23.449557

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