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LUCas: Light-Uncaged Cas13a using photocleavable interfering guide RNAs

2026-02-04

Abstract excerpt

CRISPR diagnostics have emerged as powerful tools for detecting infectious diseases, with the RNA endonuclease Cas13a enabling sensitive and specific, amplification-free RNA detection through collateral trans -cleavage of fluorescent reporters. However, background cleavage from unbound enzyme, contaminating nucleases, and unsynchronized initiation of reactions limits assay sensitivity and interpretability. A stra...

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Identifiers and source

Literature Corpus work
3ff621a6-27ea-5104-8677-762b7cbd1a2e
DOI
10.64898/2026.02.02.700737
Open publication

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LUCas: Light-Uncaged Cas13a using photocleavable interfering guide RNAsDOI 10.64898/2026.02.02.700737
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