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Enhancement of CRISPR-Cas12a system through universal circular RNA design

1 Jun 2025

Abstract excerpt

Precise control of Cas12a activity is crucial to address incompatibility in isothermal amplification-CRISPR-Cas12a one-pot nucleic acid detection. We developed a light-triggerable circular RNA system for dynamic LbCas12a regulation. By employing circular CRISPR guide RNA (crRNA) or a split circular universal direct repeat region with a replaceable spacer, we resolved the incompatibility between isothermal...

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PMID: 40527291
DOI: 10.1016/j.crmeth.2025.101076

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