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A photocontrolled one-pot isothermal amplification and CRISPR-Cas12a assay for rapid detection of SARS-CoV-2 Omicron variants

6 Feb 2024

Abstract excerpt

CRISPR-Cas technology has widely been applied to detect single-nucleotide mutation and is considered as the next generation of molecular diagnostics. We previously reported the combination of nucleic acid amplification (NAA) and CRISPR-Cas12a system to distinguish major severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants. However, the mixture of NAA and CRISPR-Cas12a reagents in one tube could...

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Advanced biosensing and bioanalysis techniques
CRISPR and Genetic Engineering
Mosquito-borne diseases and control

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 38319081
DOI: 10.1128/spectrum.03645-23

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