Article

Engineering protein-specific proteases: targeting active RAS

2020-09-21

Abstract excerpt

We describe the design, kinetic properties, and structures of engineered subtilisin proteases that degrade the active form of RAS by cleaving a conserved sequence in switch 2. RAS is a signaling protein that, when mutated, drives a third of human cancers. To generate high specificity for the RAS target sequence, the active site was modified to be dependent on a cofactor (imidazole or nitrite) and protease sub-site...

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Literature Corpus work: 3b03f4ff-d936-54f5-9380-126796574fc9
DOI: 10.1101/2020.09.21.303917

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