High-throughput Activity Reprogramming of Proteases (HARP)

Abstract excerpt

Developing potent and selective protease inhibitors remains a grueling, iterative, and often unsuccessful endeavor. Although macromolecular inhibitors can achieve single-enzyme specificity, platforms used for macromolecular inhibitor discovery are optimized for high-affinity binders, requiring extensive downstream biochemical characterization to isolate rare inhibitors. Here, we developed the High-throughput Activ...

Topics

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• Cell Separation
• Combinatorial Chemistry Techniques
• Endopeptidases
• Endoplasmic Reticulum
• Flow Cytometry
• Protein Engineering
• Single-Cell Analysis
• Substrate Specificity

Identifiers and source

Literature Corpus work

470e70b6-51c5-5db5-9b13-69cebdc14a39

DOI

10.1101/2025.03.27.640893