A new library of alpha-lytic protease S1 mutants generated by combinatorial random substitution.

Article

A new library of alpha-lytic protease S1 mutants generated by combinatorial random substitution.

Biochemistry and molecular biology international - 1 Apr 1994

Graham L D, Haggett K D, Hayes P J, Schober P A, Jennings P A, Whittaker R G

Abstract excerpt

Four positions in the S1 site of alpha-lytic protease (positions 190, 192, 213 and 218) were subjected to targeted random mutagenesis. In the resulting library we found active mutant proteases whose cleavage preferences could be grouped into three distinct families. Some potentially useful enzyme...

Topics

Amino Acid Sequence
Base Sequence
Gene Library
Molecular Sequence Data
Mutation
Serine Endopeptidases
Substrate Specificity

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