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Article

Evaluation of phage display system and leech-derived tryptase inhibitor as a tool for understanding the serine proteinase specificities.

Archives of biochemistry and biophysics - 1 May 2004

Campos Ivan T N, Silva Melissa M, Azzolini Simone S, Souza Adriana F, Sampaio Claudio A M, Fritz Hans, Tanaka Aparecida S

Abstract excerpt

A small combinatorial library of LDTI mutants (5.2 x 10(4)) restricted to the P1-P4' positions of the reactive site was displayed on the pCANTAB 5E phagemid, and LDTI fusion phages were produced and selected for potent neutrophil elastase and plasmin inhibitors. Strong fusion phage binders were analyzed by ELISA on enzyme-coated microtiter plates and the positive phages had their DNA sequenced. The LDTI variants:...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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PMID: 15081897
DOI: 10.1016/j.abb.2004.03.004

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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