Systematic mapping of chimera-permissive sites by CRISPR-guided PAM scanning

Abstract excerpt

Engineered protein chimeras enable new biological functions but remain difficult to design due to context-dependent constraints on insertion tolerance and the need to preserve host protein function. Here, we report CRISPR-guided protospacer adjacent motif (PAM) scanning in yeast to map chimera-permissive sites in living cells. We apply this approach to peptide and reporter insertions. In the first application, we...

Topics

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• INDEL Mutation
• CRISPR-Associated Protein 9
• Endonucleases
• Gene Knockout Techniques
• HEK293 Cells
• High-Throughput Screening Assays
• Nucleotide Motifs
• Transcriptional Activation

Identifiers and source

Literature Corpus work

eb0b01db-805b-5aa5-9f8a-0faa4ab0e333

DOI

10.64898/2026.05.06.723292