Article

Accurate characterization of CRISPR-Cas9 genome editing outcomes and mosaicism with near-perfect long reads

2025-09-09

Abstract excerpt

<h4>Background: </h4> Genetic mosaicism is a consequence of CRISPR-Cas9 genome editing that is difficult to study, especially when it involves structural variants occurring at low frequency. A comprehensive analysis of mosaicism requires deep and unbiased sequencing of the target loci, with accurate single molecule reads. Here we performed amplification-free PureTarget PacBio sequencing to investigate CRISPR-Cas9...

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Literature Corpus work: 3a003056-8114-5484-bbde-882ac252cc78
DOI: 10.1101/2025.09.08.674810

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