Article
Long-read sequencing for fast and robust identification of correct genome-edited alleles: PCR-based and Cas9 capture methods.
PLoS genetics - 1 Mar 2024
McCabe Christopher V, Price Peter D, Codner Gemma F, Allan Alasdair J, Caulder Adam, Christou Skevoulla, Loeffler Jorik, Mackenzie Matthew, Malzer Elke, Mianné Joffrey, Nowicki Krystian J, O'Neill Edward J, Pike Fran J, Hutchison Marie, Petit-Demoulière Benoit, Stewart Michelle E, Gates Hilary, Wells Sara, Sanderson Nicholas D, Teboul Lydia
Abstract excerpt
BACKGROUND: Recent developments in CRISPR/Cas9 genome-editing tools have facilitated the introduction of precise alleles, including genetic intervals spanning several kilobases, directly into the embryo. However, the introduction of donor templates, via homology directed repair, can be erroneous or incomplete and these techniques often produce mosaic founder animals. Thus, newly generated alleles must be verified...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.