CRISPR-Cas9-induced genetic mosaicism in three species of the microcrustacean Daphnia.

Abstract excerpt

Genetic mosaicism can arise from in vivo CRISPR-Cas9 gene editing, especially in the embryos. This study evaluates the extent of genetic mosaicism resulted from CRISPR-Cas9-mediated knockout for 11 genes in the freshwater microcrustacean Daphnia magna, Daphnia pulex, and Daphnia sinensis. Based on extensive genotyping data of the asexually produced progenies of successfully edited females, we find strong evidence...

Topics

• Animals
• Mosaicism
• CRISPR-Cas Systems
• Daphnia
• Female
• Gene Editing
• Daphnia magna
• Daphnia pulex