Article
Enhanced prime editing systems by manipulating cellular determinants of editing outcomes.
Cell - 28 Oct 2021
Chen Peter J, Hussmann Jeffrey A, Yan Jun, Knipping Friederike, Ravisankar Purnima, Chen Pin-Fang, Chen Cidi, Nelson James W, Newby Gregory A, Sahin Mustafa, Osborn Mark J, Weissman Jonathan S, Adamson Britt, Liu David R
Abstract excerpt
While prime editing enables precise sequence changes in DNA, cellular determinants of prime editing remain poorly understood. Using pooled CRISPRi screens, we discovered that DNA mismatch repair (MMR) impedes prime editing and promotes undesired indel byproducts. We developed PE4 and PE5 prime editing systems in which transient expression of an engineered MMR-inhibiting protein enhances the efficiency of...
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