Article

Iterative engineering of a compact Cas9 ortholog for <i>in vivo</i> gene editing via single AAV delivery

2026-01-12

Abstract excerpt

<h4>ABSTRACT</h4> The development of compact and efficient CRISPR-Cas systems is crucial for biomedical and therapeutic genome editing, particularly in vivo applications based on viral delivery. Here, we performed a comparative functional screen of seven Cas9 orthologs to systematically evaluate their genome editing activity in mammalian cells. Among these, Cme2—a 1008 amino acid nuclease recognizing a 5′-NAGNGC...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 7a691664-a555-57f2-813b-ad9e12a5c581
DOI: 10.64898/2026.01.12.698990

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Iterative engineering of a compact Cas9 ortholog for <i>in vivo</i> gene editing via single AAV deliveryDOI 10.64898/2026.01.12.698990Read

Engineered Un1Cas12f1 for multiplex genome editing with enhanced activity and targeting scope.Semantic proximity Read

Compact type II-D Cas9 nucleases for efficient and specific genome editingSemantic proximity Read

Precision Cas9 Genome Editing <i>in vivo</i> with All-in-one, Self-targeting AAV VectorsSemantic proximity Read

Efficient Genome Editing with Chimeric Oligonucleotide-Directed EditingSemantic proximity Read

Engineering Nme2Cas9 Adenine Base Editors with Improved Activity and Targeting ScopeSemantic proximity Read

All-in-One Adeno-associated Virus Delivery and Genome Editing by <i>Neisseria meningitidis</i> Cas9 <i>in vivo</i>Semantic proximity Read

An engineered Cas12i nuclease that is an efficient genome editing tool in animals and plantsSemantic proximity Read

μCas, a novel class of miniature type-V Cas12f nucleases with diverse PAMSemantic proximity Read

Directed evolution of compact RNA-guided nucleases for enhanced activity in mammalian cellsSemantic proximity Read

Mutational rescue of the activity of high-fidelity Cas9 enzymes.Semantic proximity Read

Rational Design of Enhanced Nme2Cas9 and Nme2 <sup>Smu</sup> Cas9 Nucleases and Base EditorsSemantic proximity Read

Closely related type II-C Cas9 orthologs recognize diverse PAMsSemantic proximity Read