Article

Harnessing RNA-based DNA repair pathways for targeted gene editing

2024-04-10

Abstract excerpt

<h4>ABSTRACT</h4> Recent studies have revealed a role for RNA in the repair of DNA double-strand breaks. Here, we show that the asymmetric DNA overhangs generated by the small TevSaCas9 dual nuclease informs a simple and robust editing strategy in human cells whereby Polθ and Rad52 are recruited to repair the double-strand break. The 2-nt, 3’ DNA overhang generated by the I-TevI nuclease domain of TevSaCas9 hybri...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 0367cd27-f66d-5e08-9695-898ac8fc1bd7
DOI: 10.1101/2024.04.09.588775

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Harnessing RNA-based DNA repair pathways for targeted gene editingDOI 10.1101/2024.04.09.588775Read

Repair of CRISPR-guided RNA breaks enables site-specific RNA editing in human cellsSemantic proximity Read

Efficient Genome Editing with Chimeric Oligonucleotide-Directed EditingSemantic proximity Read

Programmable multi-kilobase RNA editing using CRISPR-mediated trans-splicingSemantic proximity Read

Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaksSemantic proximity Read

RGEN Editing of RNA and DNA: The Long and Winding Road from Catalytic RNAs to CRISPR to the Clinic.Semantic proximity Read

Targeted RNA editing by direct delivery of an adenosine deaminase-antisense oligo conjugateSemantic proximity Read

RNA Transcripts Serve as a Template for Double-Strand Break Repair in Human CellsSemantic proximity Read

Compact adenine base editors to enable therapeutic rescue of Duchenne muscular dystrophySemantic proximity Read

A step by step protocol for genomic knock-in of long sequences using dCas9-SSAP editorSemantic proximity Read

Recent Advances in Double-Strand Break-Free Kilobase-Scale Genome Editing Technologies.Semantic proximity Read

Methods and techniques enabling multi-kilobase long-range genomic rewrite/replace editingSemantic proximity Read

μCas, a novel class of miniature type-V Cas12f nucleases with diverse PAMSemantic proximity Read