Article

Engineering Nme2Cas9 Adenine Base Editors with Improved Activity and Targeting Scope

2023-04-14

Abstract excerpt

Nme2Cas9 has been established as a genome editing platform with compact size, high accuracy, and broad targeting range, including single-AAV-deliverable adenine base editors. Here, we have engineered Nme2Cas9 to further increase the activity and targeting scope of compact Nme2Cas9 base editors. We first used domain insertion to position the deaminase domain nearer the displaced DNA strand in the target-bound compl...

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Literature Corpus work: ee630cc1-64ee-5884-9008-964d1aae64aa
DOI: 10.1101/2023.04.14.536905

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