Article
Creation of mutant mice with megabase-sized deletions containing custom-designed breakpoints by means of the CRISPR/Cas9 system.
Scientific reports - 3 Mar 2017
Kato Tomoko, Hara Satoshi, Goto Yuji, Ogawa Yuya, Okayasu Haruka, Kubota Souichirou, Tamano Moe, Terao Miho, Takada Shuji
Abstract excerpt
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a useful tool for creation of mutant mice with mutations mirroring those in human patients. Various methods have been developed for this purpose, including deletions, inversions, and translocations. So far, mutant mice with deletions of up to 1.2 megabases (Mb) have been generated by microinjection...
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