Article

CRISPR-Cas9 HDR Optimization: RAD52, Denatured and 5’-Modified DNA Templates in Knock-In Mice Generation

2025-05-07

Abstract excerpt

<h4> Abstract </h4> CRISPR/Cas9-mediated genome editing is a powerful tool for producing animal models of human diseases. However, it often encounters challenges related to low efficiency of donor DNA templates insertion through homology-directed repair (HDR) pathway or unwanted insertions and/or multiplications. Here, we present findings from multiple targeting experiments aimed at generating a Nup93 conditiona...

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Literature Corpus work: 68d42716-cca9-53c2-b6ce-3b5f63807b8e
DOI: 10.1101/2025.05.05.652162

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