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MOSAIC enables <i>in situ</i> saturation mutagenesis of genes and CRISPR prime editing guide RNA optimization in human cells

2024-04-25

Abstract excerpt

CRISPR prime editing offers unprecedented versatility and precision for the installation of genetic edits in situ . Here we describe the development and characterization of the Multiplexing Of Site-specific Alterations for In situ Characterization ( MOSAIC ) method, which leverages a non-viral PCR-based prime editing method to enable rapid installation of thousands of defined edits in pooled fashion. We show th...

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Literature Corpus work
2c0aa1be-5bd1-5283-81cf-8db75cb4058f
DOI
10.1101/2024.04.25.591078
Open publication

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MOSAIC enables <i>in situ</i> saturation mutagenesis of genes and CRISPR prime editing guide RNA optimization in human cellsDOI 10.1101/2024.04.25.591078
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