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Article

Uncovering functional variants using a high-efficiency PE3-based screening platform.

Science advances - 5 Jun 2026

Huang Shisheng, Tao Wanyu, Li Xiangyang, Guo Junfan, Zhang Yu, Li Guanglei, Zhang Pengfei, Zhang Guiquan, Li Gaoyang, Fan Gaofeng, Zhang Shang-Min, Huang Xingxu

Abstract excerpt

Prime editing (PE) enables precise nucleotide changes but has limited utility in high-throughput functional screening due to low editing efficiency. Here, we developed EvoPRIME, a PE3-based screening platform that integrates Csy4-mediated processing of Pol II-driven guide RNAs with a fluorescence-based enrichment strategy. EvoPRIME achieves comparable efficiency to state-of-the-art prime editing systems without...

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Identifiers

PMID: 42247518
DOI: 10.1126/sciadv.ady7359
PMCID: PMC13240188

Genes

EGFR
GAB1

Variants

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

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