Article

Engineering oncogenic hotspot mutations on <i>SF3B1</i> via CRISPR-directed PRECIS mutagenesis

2024-02-25

Abstract excerpt

SF3B1 is the most recurrently mutated RNA splicing factor in cancer; However, its study has been hindered by a lack of disease-relevant cell line models. Here, we compared four genome engineering platforms to establish SF3B1 mutant cell lines: CRISPR-Cas9 editing, AAV HDR editing, base editing (ABEmax, ABE8e), and prime editing (PE2, PE3, PE5Max). We showed that prime editing via PE5max achieved the most efficien...

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Identifiers and source

Literature Corpus work: 7c04c05c-b855-5998-b54c-887e117afd19
DOI: 10.1101/2024.02.23.581842

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