Article

Scalable Design of paired CRISPR Guide RNAs for Genomic Deletion

2016-05-21

Abstract excerpt

Using CRISPR/Cas9, diverse genomic elements may be studied in their endogenous context. Pairs of single guide RNAs (sgRNAs) are used to delete regulatory elements and small RNA genes, while longer RNAs can be silenced through promoter deletion. We here present CRISPETa, a bioinformatic pipeline for flexible and scalable paired sgRNA design based on an empirical scoring model. Multiple sgRNA pairs are returned for...

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Literature Corpus work: a79eefd5-28e6-5d07-9647-9cfc9d22a67e
DOI: 10.1101/052795

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