Article

Fine-tuning of substrate preferences of the Src-family kinase Lck revealed through a high-throughput specificity screen

2018-01-19

Abstract excerpt

To obtain a comprehensive map of the intrinsic specificities of tyrosine kinase domains, we developed a high-throughput method that uses bacterial surface-display and next-generation sequencing to analyze the specificity of any tyrosine kinase against a library of thousands of peptides derived from human tyrosine phosphorylation sites. Using this approach, we identified a difference in the electrostatic recognitio...

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Identifiers and source

Literature Corpus work: 26cf1e0e-30b6-52c7-9313-f1ed3855d9a1
DOI: 10.1101/250753

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