Article

Base editing using CRISPR/Cas9 in <i>Drosophila</i>

2021-03-25

Abstract excerpt

Cas9 and a guide RNA function to target specific genomic loci for generation of a double stranded break. Catalytic dead versions of Cas9 (dCas9) no longer cause double stranded breaks and instead can serve as molecular scaffolds to target additional enzymatic proteins to specific genomic loci. To generate mutations in selected genomic residues, dCas9 can be used for genomic base editing by fusing a cytidine deamin...

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Literature Corpus work: 556df2c6-bc37-50f9-b774-c6c08c8ce7b7
DOI: 10.1101/2021.03.24.436868

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