Article

Enhance genome editing efficiency and specificity by a quick CRISPR/Cas9 system

2019-08-02

Abstract excerpt

CRISPR/Cas9 is a powerful genome editing tool that has been successfully applied to a variety of species, including zebrafish. However, targeting efficiencies vary greatly at different genomic loci, the underlying causes of which were still elusive. Here we report a quick CRISPR/Cas9 system, designated as qCas9, which exhibits accelerated turnover of Cas9 protein in zebrafish. Our data showed that qCas9 significan...

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Literature Corpus work: 3414aa36-f2a0-55fa-9bd2-7ab2557f083b
DOI: 10.1101/708404

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