Article
A natural single-guide RNA repurposes Cas9 to autoregulate CRISPR-Cas expression.
Cell - 4 Feb 2021
Workman Rachael E, Pammi Teja, Nguyen Binh T K, Graeff Leonardo W, Smith Erika, Sebald Suzanne M, Stoltzfus Marie J, Euler Chad W, Modell Joshua W
Abstract excerpt
CRISPR-Cas systems provide prokaryotes with acquired immunity against viruses and plasmids, but how these systems are regulated to prevent autoimmunity is poorly understood. Here, we show that in the S. pyogenes CRISPR-Cas system, a long-form transactivating CRISPR RNA (tracr-L) folds into a natural single guide that directs Cas9 to transcriptionally repress its own promoter (Pcas). Further, we demonstrate that...
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