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Article

Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologs.

Nucleic acids research - 4 Jun 2020

Jacobsen Thomas, Ttofali Fani, Liao Chunyu, Manchalu Srinivas, Gray Benjamin N, Beisel Chase L

Abstract excerpt

CRISPR-Cas systems comprise diverse adaptive immune systems in prokaryotes whose RNA-directed nucleases have been co-opted for various technologies. Recent efforts have focused on expanding the number of known CRISPR-Cas subtypes to identify nucleases with novel properties. However, the functional diversity of nucleases within each subtype remains poorly explored. Here, we used cell-free transcription-translation...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 32329776
DOI: 10.1093/nar/gkaa272
PMCID: PMC7261169

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