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High-throughput CRISPRi phenotyping in <i>Streptococcus pneumoniae</i> identifies new essential genes involved in cell wall synthesis and competence development

2016-11-17

Abstract excerpt

Genome-wide screens have discovered a large set of essential genes in the opportunistic human pathogen Streptococcus pneumoniae . However, the functions of many essential genes are still unknown, hampering vaccine development and drug discovery. Based on results from transposon-sequencing (Tn-Seq), we refined the list of essential genes in S. pneumoniae serotype 2 strain D39. Next, we created a knockdown library...

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Literature Corpus work
18b8bd98-e406-574a-8c21-647625b690fe
DOI
10.1101/088336
Open publication

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High-throughput CRISPRi phenotyping in <i>Streptococcus pneumoniae</i> identifies new essential genes involved in cell wall synthesis and competence developmentDOI 10.1101/088336
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