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High‐throughput CRISPRi phenotyping identifies new essential genes in Streptococcus pneumoniae

1 May 2017

Abstract excerpt

Abstract Genome‐wide screens have discovered a large set of essential genes in the opportunistic human pathogen Streptococcus pneumoniae . However, the functions of many essential genes are still unknown, hampering vaccine development and drug discovery. Based on results from transposon sequencing (Tn‐seq), we refined the list of essential genes in S. pneumoniae serotype 2 strain D39. Next, we created a knockdown...

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PMID: 28490437
DOI: 10.15252/msb.20167449

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