Back to search

Article

<i>In vivo</i> genome-wide CRISPRi-Seq in <i>Streptococcus pneumoniae</i> reveals host-adaptive pathways essential for meningitis and identifies new therapeutic targets

2025-11-01

Abstract excerpt

Streptococcus pneumoniae is an important human pathogen that causes invasive diseases and remains the leading cause of bacterial meningitis. However, the bacterial determinants required for survival within the central nervous system (CNS) are poorly understood. Here, we performed a genome-wide CRISPR interference screen (CRISPRi-Seq) in an in vivo zebrafish model to systematically map pneumococcal fitness determi...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
07c3484e-e17e-57df-8bfe-5937641fb168
DOI
10.1101/2025.10.31.685840
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
<i>In vivo</i> genome-wide CRISPRi-Seq in <i>Streptococcus pneumoniae</i> reveals host-adaptive pathways essential for meningitis and identifies new therapeutic targetsDOI 10.1101/2025.10.31.685840
Exploration of bacterial bottlenecks and <i>Streptococcus pneumoniae</i> pathogenesis by CRISPRi-seqSemantic proximity
LacR and TrxB are key virulence factors involved in pneumococcal meningitis as identified by a genome-wide association studySemantic proximity
High-throughput CRISPRi phenotyping in <i>Streptococcus pneumoniae</i> identifies new essential genes involved in cell wall synthesis and competence developmentSemantic proximity
<i>Pseudomonas aeruginosa</i> essential gene perturbations that confer vulnerability to the mammalian host environmentSemantic proximity
A functional genetic landscape of antibiotic sensitivity across the pneumococcal pangenome reveals conserved and lineage-specific vulnerabilitiesSemantic proximity
A genome-wide atlas of antibiotic susceptibility targets and pathways to toleranceSemantic proximity
A genome-wide atlas of antibiotic susceptibility targets and pathways to toleranceSemantic proximity
High‐throughput CRISPRi phenotyping identifies new essential genes in Streptococcus pneumoniaeSemantic proximity
<i>Klebsiella pneumoniae</i> causes bacteremia using factors that mediate tissue-specific fitness and resistance to oxidative stressSemantic proximity
Pneumococcal metabolic adaptation and colonization is regulated by the two-component regulatory system 08Semantic proximity
From the Gut to the Brain: Transcriptomic Insights into Neonatal Meningitis Escherichia coli Across Diverse Host NichesSemantic proximity
Chemical genomics informs antibiotic and essential gene function in <i>Acinetobacter baumannii</i>Semantic proximity
Select a neighboring publication to make it the new centre.