Article

Exploration of bacterial bottlenecks and <i>Streptococcus pneumoniae</i> pathogenesis by CRISPRi-seq

2020-04-23

Abstract excerpt

Streptococcus pneumoniae is a commensal bacterium of the human nasopharynx, but can cause harmful infections if it spreads to other parts of the body, such as pneumonia, sepsis or meningitis. To facilitate pathogenesis studies, we constructed a doxycycline-inducible pooled CRISPR interference (CRISPRi) library targeting all operons in protypical S. pneumoniae strain D39V. Our library design allows fitness within...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: c0e559fb-e096-5370-9669-23d9f2fb08c9
DOI: 10.1101/2020.04.22.055319

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Exploration of bacterial bottlenecks and <i>Streptococcus pneumoniae</i> pathogenesis by CRISPRi-seqDOI 10.1101/2020.04.22.055319Read

High-throughput CRISPRi phenotyping in <i>Streptococcus pneumoniae</i> identifies new essential genes involved in cell wall synthesis and competence developmentSemantic proximity Read

High‐throughput CRISPRi phenotyping identifies new essential genes in Streptococcus pneumoniaeSemantic proximity Read

Dual CRISPRi-Seq for genome-wide genetic interaction studies identifies key genes involved in the pneumococcal cell cycleSemantic proximity Read

<i>In vivo</i> genome-wide CRISPRi-Seq in <i>Streptococcus pneumoniae</i> reveals host-adaptive pathways essential for meningitis and identifies new therapeutic targetsSemantic proximity Read

Mobile-CRISPRi: Enabling Genetic Analysis of Diverse BacteriaSemantic proximity Read

<i>Pseudomonas aeruginosa</i> essential gene perturbations that confer vulnerability to the mammalian host environmentSemantic proximity Read

An efficient <i>in vivo</i> -inducible CRISPR interference system for group A <i>Streptococcus</i> genetic analysis and pathogenesis studiesSemantic proximity Read

An efficient in vivo -inducible CRISPR interference system for group A Streptococcus genetic analysis and pathogenesis studiesSemantic proximity Read

A CRISPRi-based genetic resource to study essential <i>Staphylococcus aureus</i> genesSemantic proximity Read

CRISPRi-seq in <i>Haemophilus influenzae</i> reveals genome-wide and medium-specific growth determinantsSemantic proximity Read

Multiplex CRISPRi-Cas9 silencing of planktonic and stage-specific biofilm genes in <i>Enterococcus faecalis</i>Semantic proximity Read

A functional genetic landscape of antibiotic sensitivity across the pneumococcal pangenome reveals conserved and lineage-specific vulnerabilitiesSemantic proximity Read