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Repurposing the Streptococcus mutans CRISPR-Cas9 System to Understand Essential Gene Function

9 Mar 2020

Abstract excerpt

A recent genome-wide screen identified ~300 essential or growth-supporting genes in the dental caries pathogen Streptococcus mutans.To be able to study these genes, we built a CRISPR interference tool around the Cas9 nuclease (Cas9 Smu ) encoded in the S. mutans UA159 genome.Using a xylose-inducible dead Cas9 Smu with a constitutively active singleguide RNA (sgRNA), we observed titratable repression of GFP...

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PMID: 32150575
DOI: 10.1371/journal.ppat.1008344

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