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Article

Directed evolution of hyperactive integrases for site specific insertion of transgenes.

Nucleic acids research - 12 Aug 2024

Hew Brian E, Gupta Sabranth, Sato Ryuei, Waller David F, Stoytchev Ilko, Short James E, Sharek Lisa, Tran Christopher T, Badran Ahmed H, Owens Jesse B

Abstract excerpt

The ability to deliver large transgenes to a single genomic sequence with high efficiency would accelerate biomedical interventions. Current methods suffer from low insertion efficiency and most rely on undesired double-strand DNA breaks. Serine integrases catalyze the insertion of large DNA cargos at attachment (att) sites. By targeting att sites to the genome using technologies such as prime editing, integrases...

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Share to Attachment Sites, Microbiological
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Directed Molecular Evolution

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Source

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Identifiers

PMID: 38953167
DOI: 10.1093/nar/gkae534
PMCID: PMC11317131

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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