CRISPR/Cas12a dual-gRNA assay enables precise detection of single-nucleotide mutations via cis-staggered-cleavage.

doi:10.1016/j.aca.2026.345968

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CRISPR/Cas12a dual-gRNA assay enables precise detection of single-nucleotide mutations via cis-staggered-cleavage.

Analytica chimica acta - 22 Oct 2026

Lin Zhiyun, Luo Yanchi, Li Hong, Shao Baokai, Yang Xiufen, Wang Jianing, Wan Yi, Song Fengge

Abstract excerpt

Robust discrimination of single-nucleotide mutations (SNMs) remains a central challenge in nucleic acid analysis, particularly under minimal sequence constraints. Here, we report a programmable CRISPR/Cas12a sensing strategy, termed STAND (cis-staggered-cleavage-based dual-gRNA assay), that enables precise SNM discrimination through a structurally gated cleavage cascade. In this system, a primary guide RNA...

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PMID: 42648842
DOI: 10.1016/j.aca.2026.345968

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