A spacer design strategy for CRISPR-Cas12f1 with single-nucleotide polymorphism mutation resolution capability and its application in the mutations diagnosis of pathogens.

doi:10.1002/jmv.29189

Article

A spacer design strategy for CRISPR-Cas12f1 with single-nucleotide polymorphism mutation resolution capability and its application in the mutations diagnosis of pathogens.

Journal of medical virology - 1 Oct 2023

Gao Panqi, Yang Maoyi, Chen Yi, Yan Jun, Han Miaomiao, Deng Haijun, Qian Keli, Yang Jiandong, Lu Yaoqin, Zhou Ling, Huang Ailong, Li Xiaosong, Deng Wanyan, Long Quanxin

Abstract excerpt

Infectious diseases remain a major global issue in public health. It is important to develop rapid, sensitive, and accurate diagnostic methods to detect pathogens and their mutations. Cas12f1 is an exceptionally compact RNA-guided nuclease and have the potential to fulfill the clinical needs. Based on the interaction between crRNA-SSDNA binary sequence and Cas12f1, here, we addressed the essential features that...

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A spacer design strategy for CRISPR-Cas12f1 with single-nucleotide polymorphism mutation resolution capability and its application in the mutations diagnosis of pathogens.

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