Article

Sensitive multimodal profiling of native DNA by transposase-mediated single-molecule sequencing

2022-08-07

Abstract excerpt

<h4>ABSTRACT</h4> We present SMRT-Tag: a multiplexable, PCR-free approach for constructing low-input, single-molecule Pacific Biosciences (PacBio) sequencing libraries through Tn5 transposition. As proof-of-concept, we apply SMRT-Tag to resolve human genetic and epigenetic variation in gold-standard human reference samples. SMRT-Tag requires 1-5% as much input material as existing protocols (15,000 – 50,000 human...

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Literature Corpus work: 147ac963-42d7-5fa4-aea1-ea7d99341005
DOI: 10.1101/2022.08.07.502893

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