Article

Sleeping ORANGE: A Transposase-Enhanced CRISPR Approach to Boost Endogenous Protein Tagging Efficiency

2025-10-23

Abstract excerpt

<h4>Background</h4> Investigating the subcellular distribution of proteins is crucial for understanding complex cell behaviours and disease mechanisms, and fluorescence microscopy has become a key tool for visualising protein localisation. Endogenous protein tagging, where the sequence for a tag (typically a peptide or fluorescent protein) is integrated into the native genetic sequence encoding a protein of inter...

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Literature Corpus work: 11a0b4c9-d43e-5faa-864d-5a56c5144bb6
DOI: 10.1101/2025.10.23.684145

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