Article

Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach

2021-07-29

Abstract excerpt

The CRISPR/Cas9 system has been used to generate fluorescently labelled fusion proteins by homology directed repair in a variety of species. Despite its revolutionary success, there remains an urgent need for increased simplicity and efficiency of genome editing in research organisms. Here, we establish a simplified, highly efficient and precise strategy for CRISPR/Cas9 mediated endogenous protein tagging in medak...

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Literature Corpus work: 54e4bb9c-3bf6-55ee-b897-aaa9685e9d24
DOI: 10.1101/2021.07.29.454295

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