Article

Endogenous tagging using split mNeonGreen in human iPSCs for live imaging studies

2023-06-28

Abstract excerpt

Endogenous tags have become invaluable tools to visualize and study native proteins in live cells. However, generating human cell lines carrying endogenous tags is difficult due to the low efficiency of homology-directed repair. Recently, an engineered split mNeonGreen protein was used to generate a large-scale endogenous tag library in HEK293 cells. Using split mNeonGreen for large-scale endogenous tagging in hum...

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Literature Corpus work: f7d9411d-f1c5-5eac-9a99-e76587882bd9
DOI: 10.1101/2023.06.28.546942

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