Article
Efficient and error-free fluorescent gene tagging in human organoids without double-strand DNA cleavage.
PLoS biology - 1 Jan 2022
Bollen Yannik, Hageman Joris H, van Leenen Petra, Derks Lucca L M, Ponsioen Bas, Buissant des Amorie Julian R, Verlaan-Klink Ingrid, van den Bos Myrna, Terstappen Leon W M M, van Boxtel Ruben, Snippert Hugo J G
Abstract excerpt
CRISPR-associated nucleases are powerful tools for precise genome editing of model systems, including human organoids. Current methods describing fluorescent gene tagging in organoids rely on the generation of DNA double-strand breaks (DSBs) to stimulate homology-directed repair (HDR) or non-homologous end joining (NHEJ)-mediated integration of the desired knock-in. A major downside associated with DSB-mediated...
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