Article

Live-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells

2020-02-28

Abstract excerpt

<h4>ABSTRACT</h4> The current model of the mammalian circadian oscillator is predominantly based on data from genetics and biochemistry experiments, while the cell biology of circadian clocks is still in its infancy. Here, we describe a new strategy for the efficient generation of knock-in reporter cell lines using CRISPR technology that is particularly useful for lowly or transiently expressed genes, such as tho...

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Literature Corpus work: 0e5d5fd6-74d3-5a60-9d30-f822eb96bb98
DOI: 10.1101/2020.02.28.967752

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