Article
Biological plasticity rescues target activity in CRISPR knock outs.
Nature methods - 1 Nov 2019
Smits Arne H, Ziebell Frederik, Joberty Gerard, Zinn Nico, Mueller William F, Clauder-Münster Sandra, Eberhard Dirk, Fälth Savitski Maria, Grandi Paola, Jakob Petra, Michon Anne-Marie, Sun Hanice, Tessmer Karen, Bürckstümmer Tilmann, Bantscheff Marcus, Steinmetz Lars M, Drewes Gerard, Huber Wolfgang
Abstract excerpt
Gene knock outs (KOs) are efficiently engineered through CRISPR-Cas9-induced frameshift mutations. While the efficiency of DNA editing is readily verified by DNA sequencing, a systematic understanding of the efficiency of protein elimination has been lacking. Here we devised an experimental strategy combining RNA sequencing and triple-stage mass spectrometry to characterize 193 genetically verified deletions...
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