Article
Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system.
PloS one - 1 Jan 2018
Szczesny Roman J, Kowalska Katarzyna, Klosowska-Kosicka Kamila, Chlebowski Aleksander, Owczarek Ewelina P, Warkocki Zbigniew, Kulinski Tomasz M, Adamska Dorota, Affek Kamila, Jedroszkowiak Agata, Kotrys Anna V, Tomecki Rafal, Krawczyk Pawel S, Borowski Lukasz S, Dziembowski Andrzej
Abstract excerpt
Deciphering a function of a given protein requires investigating various biological aspects. Usually, the protein of interest is expressed with a fusion tag that aids or allows subsequent analyses. Additionally, downregulation or inactivation of the studied gene enables functional studies. Development of the CRISPR/Cas9 methodology opened many possibilities but in many cases it is restricted to non-essential...
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