Article

A bulk cell heterozygous knock-in strategy for targeted protein degradation

2026-05-21

Abstract excerpt

Targeted protein degradation using conditional degron tag (CDT) technology is a powerful method for rapidly degrading a protein of interest (POI) upon the addition of a degrader drug. A prerequisite for the temporally controlled degradation of an endogenous POI is the generation of homozygous knock-in cells with the degron tag integrated at either the N- or C-terminus of their gene loci. However, obtaining those h...

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Literature Corpus work: bf207ac9-a36a-57bc-bb26-4e8dc8bd19b2
DOI: 10.64898/2026.05.19.726384

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