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Bidirectional epigenetic editing reveals hierarchies in gene regulation

17 May 2024

Abstract excerpt

CRISPR perturbation methods are limited in their ability to study non-coding elements and genetic interactions. In this study, we developed a system for bidirectional epigenetic editing, called CRISPRai, in which we apply activating (CRISPRa) and repressive (CRISPRi) perturbations to two loci simultaneously in the same cell. We developed CRISPRai Perturb-seq by coupling dual perturbation gRNA detection with...

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PMID: 38760566
DOI: 10.1038/s41587-024-02213-3

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