Article

Activity-by-Contact model of enhancer specificity from thousands of CRISPR perturbations

2019-01-26

Abstract excerpt

Mammalian genomes harbor millions of noncoding elements called enhancers that quantitatively regulate gene expression, but it remains unclear which enhancers regulate which genes. Here we describe an experimental approach, based on CRISPR interference, RNA FISH, and flow cytometry (CRISPRi-FlowFISH), to perturb enhancers in the genome, and apply it to test >3,000 potential regulatory enhancer-gene connections acro...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: b156de3e-2682-58e9-9a6b-3db6626bc6be
DOI: 10.1101/529990

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Activity-by-Contact model of enhancer specificity from thousands of CRISPR perturbationsDOI 10.1101/529990Read

An encyclopedia of enhancer-gene regulatory interactions in the human genomeSemantic proximity Read

Mapping enhancer-gene regulatory interactions from single-cell dataSemantic proximity Read

Genome-wide maps of enhancer regulation connect risk variants to disease genesSemantic proximity Read

Dynamic network-guided CRISPRi screen reveals CTCF loop-constrained nonlinear enhancer-gene regulatory activity in cell state transitionsSemantic proximity Read

Mammalian enhancers and GWASs act proximally and seldom skip active genesSemantic proximity Read

Massively parallel reporter assay–informed modeling improves prediction of context-specific enhancer–gene regulatory interactionsSemantic proximity Read

Enhancer-gene regulatory interactions in colorectal cancer revealed through genome-wide CRISPRi perturbationsSemantic proximity Read

A systematic survey of distal element-gene regulatory interactions with Direct-Capture Targeted Perturb-seqSemantic proximity Read

Genome-wide enhancer maps link risk variants to disease genes.Semantic proximity Read

crisprQTL mapping as a genome-wide association framework for cellular genetic screensSemantic proximity Read

A mathematical model clarifies the ABC Score formula used in enhancer-gene predictionSemantic proximity Read

Coupled single-cell CRISPR screening and epigenomic profiling reveals causal gene regulatory networksSemantic proximity Read