Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Target-enriched nanopore sequencing and de novo assembly reveals co-occurrences of complex on-target genomic rearrangements induced by CRISPR-Cas9 in human cells.

Genome research - 1 Oct 2022

Geng Keyi, Merino Lara G, Wedemann Linda, Martens Aniek, Sobota Małgorzata, Sanchez Yerma P, Søndergaard Jonas Nørskov, White Robert J, Kutter Claudia

Abstract excerpt

The CRISPR-Cas9 system is widely used to permanently delete genomic regions via dual guide RNAs. Genomic rearrangements induced by CRISPR-Cas9 can occur, but continuous technical developments make it possible to characterize complex on-target effects. We combined an innovative droplet-based target enrichment approach with long-read sequencing and coupled it to a customized de novo sequence assembly. This approach...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alleles
Share to CRISPR-Cas Systems

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genomics

Share to Nanopore Sequencing

Share to RNA, Guide, CRISPR-Cas Systems

Source

Open in PubMed Open full text

Identifiers

PMID: 36180232
DOI: 10.1101/gr.276901.122
PMCID: PMC9712622

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Target-enriched nanopore sequencing and de novo assembly reveals co-occurrences of complex on-target genomic rearrangements induced by CRISPR-Cas9 in human cells.PMID 36180232Read ↗

CRISPR/Cas9 deletions induce adverse on-target genomic effects leading to functional DNA in human cellsSemantic proximity Read ↗

GREPore-seq: A Robust Workflow to Detect Changes after Gene Editing through Long-range PCR and Nanopore SequencingSemantic proximity Read ↗

Cas9-Assisted Targeting of CHromosome segments (CATCH) for targeted nanopore sequencing and optical genome mappingSemantic proximity Read ↗

IVT generation of guideRNAs for Cas9-enrichment Nanopore SequencingSemantic proximity Read ↗

Genome-wide structural variant landscape following HDR-enhanced CRISPR editing in human hematopoietic stem and progenitor cellsSemantic proximity Read ↗

Analyzing long-read CRISPR experiments with CRISPRLungoSemantic proximity Read ↗

Global detection of DNA repair outcomes induced by CRISPR-Cas9Semantic proximity Read ↗

CRISPR-CAS9 D10A nickase target-specific fluorescent labeling of double strand DNA for whole genome mapping and structural variation analysis.Semantic proximity Read ↗

Comprehensive analysis and accurate quantification of unintended large gene modifications induced by CRISPR-Cas9 gene editingSemantic proximity Read ↗

Massively Targeted Evaluation of Therapeutic CRISPR Off-Targets in CellsSemantic proximity Read ↗

Targeted Nanopore Sequencing with Cas9 for studies of methylation, structural variants, and mutationsSemantic proximity Read ↗

CRISPR-based platforms for detecting tumor-associated genetic materials in clinical samples.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.