Article
Prime editing for functional repair in patient-derived disease models
2020-06-10
Abstract excerpt
Prime editing is a novel genome editing technology using fusion proteins of Cas9-nickase and reverse transcriptase, that holds promise to correct the vast majority of genetic defects. We develop prime editing for primary adult stem cells grown in organoid culture models. First, we generate precise in-frame deletions in the gene encoding ß-catenin ( CTNNB1 ) that result in proliferation independent of Wnt-stimuli,...
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Identifiers and source
- Literature Corpus work
- 23573189-bace-5d07-828e-49cdf980b517
- DOI
- 10.1101/2020.06.09.139782