Article
Report from the HLA class II typing by PCR-SSP Multicentre Study.
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Jun 1997
Zetterquist H, Bengtsson M, Bäckström G, Egle-Jansson I, Ekdahl A-M, Grunnet N, Gustafsson I, Knutsen I, Kuhle A, Rydberg L, Spurkland A, Steffensen R, Storgärds M, Szojmer E, Söderholm G, Thuresson B, Turesson H, Olerup O
Abstract excerpt
Results from 360 HLA-DR and -DQ 'low-resolution' typings with polymerase chain reaction sequence-specific primers (PCR-SSP), performed by nine laboratories, were analysed for their overall utility in routinely defining the HLA-DR1-DR18, DR51-DR53 and DQ1-DQ9 specificities in less than 2.5 h. Thirty EDTA blood samples and 10 DNA samples were distributed and analysed by each laboratory. DNA was extracted using a...
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